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Bodily mutagenesis is an efficient mutational breeding methodology for enhancing varied morphological traits of decorative crops. The experiment was undertaken to research the impact of assorted dosages of gamma irradiation on seed germination of Catharanthus roseus (Madagascar periwinkle). The experiment was performed at UCIARS, Weligatta to search out out the suitable gamma dosages for choosing planting supplies for C. roseus with the aim of crop enchancment beneath gamma irradiation. Wholesome matured dry seeds have been uncovered to 60 CO Gamma irradiation supply at Horticultural Crops Analysis and Growth Institute (HORDI), Gannoruwa, Sri Lanka. Remedies included six completely different gamma dosages similar to (T0-control) 0 Gy, (T1) 100 Gy, (T2) 200 Gy, (T3) 300 Gy, (T4) 400 Gy and (T5) 500 Gy.
A Utterly Randomized Design with 4 replicates and twelve samples per replicate was employed beneath shade home situations. Knowledge on germination have been collected every day till fixed germination was noticed for as much as 10 days, whereas different administration practices have been maintained. Knowledge have been analyzed utilizing Minitab 17 statistical software program with one-way ANOVA and Tukey’s check to establish important variations amongst therapies at a 0.05 significance degree. The outcomes revealed that the upper dosages of 60 CO Gamma irradiation considerably decreased the germination skill of C. roseus seeds (P<0.05). Particularly, 400 Gy and 500 Gy therapies resulted in important reductions in germination, whereas decrease dosages lower than 400 Gy didn’t exhibit such results. Seed germination began on day 3 after planting for all therapies aside from T5 (500 Gy). Indicating a delayed germination initiation, seeds subjected to the T5 therapy started germination on day 5 after planting and exhibited a considerably decrease general germination proportion when in comparison with different therapies.
The very best germination fee (84%) was noticed within the management seeds, which weren’t subjected to gamma irradiation, whereas the bottom germination fee (8%) occurred in seeds handled with 500 Gy of gamma irradiation. Excessive doses of gamma irradiation negatively impression germination by doubtlessly inducing oxidative stress, which results in mobile harm and diminished germination effectivity. At increased doses, similar to 400 Gy and 500 Gy (as in T4 and T5 therapies respectively) radiation may cause important harm to the DNA and mobile membranes, resulting in delayed germination initiation and diminished general germination percentages. These findings have sensible implications for using gamma irradiation in plant breeding and seed preservation, emphasizing the necessity to rigorously optimize irradiation doses to keep away from adversarial results on seed germination of C. roseus.
Sriwarnsinghe, Nelka & Vidanapthirana, Nisansala & Silva, Tara & Subasinghe, Siripala & Dahanayake, Nilanthi. (2024). EFFECT OF GAMMA IRRADIATION ON SEED GERMINATION OF Catharanthus roseus (MADAGASCAR PERIWINKLE).
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