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Aneuploids are helpful supplies of genetic variety for genetic evaluation and enchancment in numerous plant species, which may be propagated primarily by way of in vitro tradition strategies. Nevertheless, somaclonal variation is frequent in tissue culture-derived crops together with euploid caladium. Within the current research, the genetic stability of in vitro-propagated crops from the leaf cultures of two kinds of caladium (Caladium × hortulanum Birdsey) aneuploids obtained beforehand was analyzed morphologically, cytologically, and molecularly.
Out of the randomly chosen 23 and eight crops regenerated from the diploid aneuploid SVT9 (2n = 2x − 2 = 28) and the tetraploid aneuploid SVT14 (2n = 4x − 6 = 54), respectively, 5 crops from the SVT9 and three crops from the SVT14 exhibited morphological variations from their corresponding dad or mum. Stomatal evaluation indicated that each the SVT9-derived variants and the SVT14-originated crops confirmed vital variations in stomatal guard cell size and width. As well as, the variants from the SVT14 have been noticed to have rounder and thicker leaves with bigger stomatal guard cells and considerably diminished stomatal density in contrast with the regenerants of the SVT9. Amongst the established crops from the SVT9, two morphological variants containing 3.14–3.58% much less imply fluorescence depth (MFI) misplaced one chromosome, and 4 variants containing 4.55–11.02% extra MFI gained one or two chromosomes. As for the crops regenerated from the SVT14, one variant with considerably larger MFI gained two chromosomes and three crops having considerably decrease MFI resulted in dropping 4 chromosomes.
Three, out of the twelve, easy sequence repeat (SSR) markers recognized DNA band profile modifications in 4 variants from the SVT9, whereas no polymorphism was detected among the many SVT14 and its regenerants. These outcomes indicated {that a} comparatively excessive frequency of somaclonal variation occurred within the in vitro-propagated crops from caladium aneuploids, particularly for the tetraploid aneuploid caladium. Newly produced aneuploid crops are extremely helpful germplasm for future genetic enchancment and analysis in caladium.
Yu, Shuangying & Zhao, Xiaoqin & Wang, Yida & Jiang, Dongzhu & Zhang, Yiming & Hu, Liu & Liu, Yiqing & Cai, Xiaodong. (2022). Morphological, Cytological, and Molecular-Primarily based Genetic Stability Evaluation of In Vitro-Propagated Crops from Newly Induced Aneuploids in Caladium. Agriculture. 12. 1708. DOI:10.3390/agriculture12101708.
Supply: ResearchGate
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